Course syllabus
Biokemi
Biochemistry
KBK011, 7,5 credits, G1 (First Cycle)
Valid for: 2016/17
Decided by: Education Board C
Date of Decision: 2016-04-12
General Information
Main field: Technology.
Compulsory for: B2
Language of instruction: The course will be given in Swedish
Aim
The aim of the course is to give basic knowledge in
biochemistry, particularly within protein chemistry, enzymology and
metabolism as well as basic abilities in biochemical laboratory
work.
Learning outcomes
Knowledge and understanding
For a passing grade the student must
- be able to explain and describe central methods for
purification and characterization of proteins
- be able to explain and describe enzyme function and
regulation
- be able to discuss and describe the three steps of energy
metabolism on an semi-advanced level
- be able to use the concept of oxidation/reduction to explain
cellular energy transformations
- be able to describe the cellular synthesis of biomolecules on a
basic level
- be able to explain, describe and generalise central concepts of
metabolic regulation
Competences and skills
For a passing grade the student must
- be able to in a group design and carry out a protein
purification process in laboratory scale
- have the ability to master some common biochemical laboratory
techniques
Judgement and approach
For a passing grade the student must
- be able to to estimate the feasibility of a method for
purifying a protein from a biological material.
- have the ability to make an overall assessment of the
nutritional contents in a food product and how this can influence
the health status of an individual.
Contents
- different methods for protein purification and protein
characterization
- examples of specific enzyme mechanisms
- carbohydrates in biological systems
- the three steps of energy metabolism
- control of metabolism by different methods
- mechanisms of hormone action
- electron transport and its relation to ATP synthesis
- biosynthesis of central biomolecules
- purification of an enzyme in a laboratory practical
Examination details
Grading scale: TH
Assessment: Written examination and a passed report from a laboratory practical.
Parts
Code: 0105. Name: Biochemistry, Theory.
Credits: 6. Grading scale: TH. Assessment: Written examination. Contents: Different methods for protein purification and protein characterization. Examples of specific enzyme mechanisms. Carbohydrates in biological systems. The three steps of energy metabolism. Control of metabolism by different methods. Mechanisms of hormone action. Electron transport and its relation to ATP synthesis. Biosynthesis of central biomolecules.
Code: 0205. Name: Biochemistry, Practicals.
Credits: 1,5. Grading scale: UG. Assessment: Passed report. Contents: The laboratory practical covers the purification of genetically modified lactate dehydrogenase from E.coli.
Admission
The number of participants is limited to: No
The course overlaps following course/s: KBK010, KBK020
Reading list
- Berg, J.M., Tymoczko, J.L., Gatto, G.J. and Stryer, L.: Biochemistry. W.H. Freeman & Co, 2015, ISBN: 978-1-4641-2610-9.
Contact and other information
Course coordinator: Professor Leif Bülow, Leif.Bulow@tbiokem.lth.se
Course homepage: http://www.tbiokem.lth.se/english/education/biochemistry/